pentr expression vector Search Results


pentr4 gateway entry vector  (New England Biolabs)
99
New England Biolabs pentr4 gateway entry vector
Pentr4 Gateway Entry Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/EcoRI-HF/10__1158_slash_1541___7786__mcr___19___1181-65-10-18
Average 99 stars, based on 1 article reviews
pentr4 gateway entry vector - by Bioz Stars, 2026-09
99/100 stars
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tgfp vector  (OriGene)
93
OriGene tgfp vector
Tgfp Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pEntry-tGFP+Mammalian+Expression+Vector/us12391748-222-7-9
Average 93 stars, based on 1 article reviews
tgfp vector - by Bioz Stars, 2026-09
93/100 stars
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pentr d topo vector  (Addgene inc)
96
Addgene inc pentr d topo vector
Pentr D Topo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/Destination+Vector+(Plasmid+%23125754)/pm39356770-199-8-20
Average 96 stars, based on 1 article reviews
pentr d topo vector - by Bioz Stars, 2026-09
96/100 stars
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yap expression vectors 79  (Addgene inc)
93
Addgene inc yap expression vectors 79
Yap Expression Vectors 79, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pENTR4-FKBP12DD+N-term+C+(w385-10)+(Plasmid+%2317416)/10__1158_slash_2767___9764__crc___24___0101-52-1-8
Average 93 stars, based on 1 article reviews
yap expression vectors 79 - by Bioz Stars, 2026-09
93/100 stars
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small rna expression vector  (Addgene inc)
91
Addgene inc small rna expression vector
Small Rna Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pENTR%2FpSM2(CMV)+GFP+(w513-1)+(Plasmid+%2319170)/pm30942430-58-33-40
Average 91 stars, based on 1 article reviews
small rna expression vector - by Bioz Stars, 2026-09
91/100 stars
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cdnas encoding sumo1  (Addgene inc)
91
Addgene inc cdnas encoding sumo1
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Cdnas Encoding Sumo1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/TRF2%CE%94B%CE%94M%2FpENTR+(Plasmid+%2319797)/pmc11550450-355-13-27
Average 91 stars, based on 1 article reviews
cdnas encoding sumo1 - by Bioz Stars, 2026-09
91/100 stars
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pentr vectors  (OriGene)
93
OriGene pentr vectors
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Pentr Vectors, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pLenti-P2A-Puro+Lentiviral+Gene+Expression+Vector/pm35715829-44-15-17
Average 93 stars, based on 1 article reviews
pentr vectors - by Bioz Stars, 2026-09
93/100 stars
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pentry  (OriGene)
96
OriGene pentry
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Pentry, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pCMV6-Entry+Mammalian+Expression+Vector/pm25721651-37-20-22
Average 96 stars, based on 1 article reviews
pentry - by Bioz Stars, 2026-09
96/100 stars
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recombinant dna dntcf4 donor vector van de wetering  (Addgene inc)
91
Addgene inc recombinant dna dntcf4 donor vector van de wetering
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Recombinant Dna Dntcf4 Donor Vector Van De Wetering, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pENTR4+TNRC6B+(Plasmid+%2320022)/pm36870059-235-168-198
Average 91 stars, based on 1 article reviews
recombinant dna dntcf4 donor vector van de wetering - by Bioz Stars, 2026-09
91/100 stars
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gateway destination vector pmk cas9 gate  (Addgene inc)
93
Addgene inc gateway destination vector pmk cas9 gate
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Gateway Destination Vector Pmk Cas9 Gate, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pENTR-PpU6P-sgRNA-L1L2+(Plasmid+%23113735)/bio_rxiv__2025__06__11__659030-226-0-4
Average 93 stars, based on 1 article reviews
gateway destination vector pmk cas9 gate - by Bioz Stars, 2026-09
93/100 stars
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pentry iba51 entry vector  (IBA Lifesciences)
90
IBA Lifesciences pentry iba51 entry vector
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Pentry Iba51 Entry Vector, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/pENTRY-IBA51+vector/bio_rxiv__077412-31-13-16
Average 90 stars, based on 1 article reviews
pentry iba51 entry vector - by Bioz Stars, 2026-09
90/100 stars
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entry vector pentr d topo ⁄ ppena1  (New England Biolabs)
95
New England Biolabs entry vector pentr d topo ⁄ ppena1
A Co-transfection of FLAG-PDHA1 and EGFP-SUMO <t>(SUMO1,</t> SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.
Entry Vector Pentr D Topo ⁄ Ppena1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+expression+vector/BclI/pm21338466-177-8-27
Average 95 stars, based on 1 article reviews
entry vector pentr d topo ⁄ ppena1 - by Bioz Stars, 2026-09
95/100 stars
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Image Search Results


A Co-transfection of FLAG-PDHA1 and EGFP-SUMO (SUMO1, SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.

Journal: NPJ Precision Oncology

Article Title: RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis

doi: 10.1038/s41698-024-00724-5

Figure Lengend Snippet: A Co-transfection of FLAG-PDHA1 and EGFP-SUMO (SUMO1, SUMO2, or SUMO3) in SW620 cells, with or without treatment of 20 μM MG132 for 6 h. Ni-NTA analyzed SUMOylation of PDHA1; B Co-transfection of FLAG-PDHA1-His, HA-Ub, and T7-SUMO3 in SW620 cells, followed by treatment with CHX (20 μg/ml), MG132 (20 μM), or CHX + MG132 for 6 h. SUMOylation and ubiquitination of PDHA1 were examined by Ni2+ pull-down assay; C Co-transfection of SW620 cells with FLAG-PDHA1-His (WT, 2 KR (UMO-deleted double mutation), K77 (SUMO-deleted single mutation), K186 (another SUMO-deleted single mutation)), HA-Ub, and T7-SUMO3, followed by treatment with 20 μM MG132 for 6 h and Ni2+ pull-down experiments to detect the SUMOylation and ubiquitination of PDHA1; D Performance of Western blot analysis to evaluate the protein expression of PDHA1, SAE1, SAE2, Ubc9, SUMO-1, and SUMO-2 in different SW620 cell groups; E Transfection of SW620 cells with Flag-PDHA1 WT, Flag-PDHA1 2 KR, Flag-PDHA1 K77, and Flag-PDHA1 K186 for 48 h. The protein expression of PDHA1 was measured by Western blot after different incubation durations with 20 μg/ml CHX. * indicates difference compared to the Flag-PDHA1 WT group, P < 0.05. Cell experiments were repeated three times.

Article Snippet: EGFP-tagged SUMO1, SUMO2, and SUMO3 were created by inserting processed forms of human cDNAs encoding SUMO1 (1-97), SUMO2 (1-92), and SUMO3 (1-93) into the pEGFP vector (165830, addgene, USA).

Techniques: Cotransfection, Ubiquitin Proteomics, Pull Down Assay, Mutagenesis, Western Blot, Expressing, Transfection, Incubation